Around 500 people from the caravan of Central American migrants began blocking El Chaparral, the main vehicle crossing point from San Diego into Tijuana, on Thursday.
Tired of waiting, the migrants decided to stage an indefinite sit-down at the entrance to the border bridge, thus hoping to exert pressure on US immigration authorities over their asylum application.
The migrants were escorted by Mexican Federal Police and personnel from the National Institute of Migration to maintain order and prevent illegal crossings.
A huge tailback of vehicles stretched into Tijuana, although the entry port still remains open to those on foot.
The situation along the border remains tense. US President Donald Trump has vowed to prevent the migrant caravan from entering the country and instead chose to bolster border security.
The migrants, mostly fleeing violence and poverty, have been travelling for over a month after the group first formed in San Pedro Sula, Honduras.